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The cast of Scrubs includes both industry veterans and newcomers to acting. They bring the wide array of Scrubs characters. The series' main cast for the first eight seasons was composed of Zach Braff, Sarah Chalke, Donald Faison, Judy Reyes, Ken Jenkins, and John C. McGinley, with Neil Flynn officially joining the main cast in the second season. In the ninth season, Eliza Coupe was upgraded to the main cast from her supporting role and Kerry Bishé, Michael Mosley, and Dave Franco joined as new main characters.

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